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Document Details
Document Type
:
Article In Journal
Document Title
:
Microbial Gutta-Percha Degradation Shares Common Steps with Rubber Degradation by Nocardia nova SH22a
Microbial Gutta-Percha Degradation Shares Common Steps with Rubber Degradation by Nocardia nova SH22a
Document Language
:
English
Abstract
:
Nocardia nova SH22a, a bacterium capable of degrading gutta-percha (GP) and natural rubber (NR), was used to investigate the GP degradation mechanism and the relations between the GP and NR degradation pathways. For this strain, a protocol of electroporation was systematically optimized, and an efficiency of up to 4.3 x 10(7) CFU per mu g of plasmid DNA was achieved. By applying this optimized protocol to N. nova SH22a, a Tn5096-based transposon mutagenesis library of this bacterium was constructed. Among about 12,000 apramycin-resistant transformants, we identified 76 stable mutants defective in GP or NR utilization. Whereas 10 mutants were specifically defective in GP utilization, the growth of the other 66 mutants was affected on both GP and NR. This indicated that the two degradation pathways are quite similar and share many common steps. The larger number of GP-degrading defective mutants could be explained in one of two ways: either (i) the GP pathway is more complex and harbors more specific steps or (ii) the steps for both pathways are almost identical, but in the case of GP degradation there are fewer enzymes involved in each step. The analysis of transposition loci and genetic studies on interesting genes confirmed the crucial role of an alpha-methylacyl-coenzyme A racemase in the degradation of both GP and NR. We also demonstrated the probable involvement of enzymes participating in oxidoreduction reactions, beta-oxidation, and the synthesis of complex cell envelope lipids in the degradation of GP.
ISSN
:
0099-2240
Journal Name
:
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Volume
:
79
Issue Number
:
4
Publishing Year
:
1434 AH
2013 AD
Article Type
:
Article
Added Date
:
Thursday, July 20, 2017
Researchers
Researcher Name (Arabic)
Researcher Name (English)
Researcher Type
Dr Grade
Email
Quan Luo
Luo, Quan
Researcher
Sebastian Hiessl
Hiessl, Sebastian
Researcher
Anja Poehlein
Poehlein, Anja
Researcher
Alexander Steinbuechel
Steinbuechel, Alexander
Researcher
Files
File Name
Type
Description
42142.pdf
pdf
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